Project B08

Local Cues Trigger Changes in the Migratory Behavior of Lymphangioblasts

The lymphatic vascular system is lined by endothelial cells and controls fluid homeostasis, fat absorption and important aspects of immune surveillance. During embryogenesis, future lymphatic endothelial cells exhibit a complex migratory behavioral pattern in both zebrafish and mice, and need to make various decisions at different points in time about which routes to take before eventually constituting a patent lymphatic vasculature network. The formation, localization, and dynamic behavior of signaling platforms between lymphatic endothelial cells and cells in their environment is to date very poorly understood. Particularly during the very first steps of lymphangiogenesis, cell-cell and cell-matrix interfaces are insufficiently studied, and the molecular and mechanistic causes for the polarized behavior of migrating lymphatic endothelial cells remain enigmatic. In the current project, we will investigate how a newly identified secreted protein, SVEP1/Polydom, governs the behavior of lymphatic endothelial cells. The SVEP1/Polydom protein, a large secreted protein that likely constitutes a component of the extracellular matrix, is expressed not by endothelial cells but rather by other cells in their immediate vicinity. Both zebrafish and mouse mutants for svep1/SVEP1 exhibit a lethal lymphatic phenotype, due to marked defects during early lymphatic cell migration. By employing a combination of in vivo imaging, immunohistochemistry, measuring actin dynamics, genetic interaction and cell culture studies, we aim to unravel how endothelial cells and neighboring cells communicate through dynamic cell-cell and cell-matrix platforms during vertebrate embryonic lymphangiogenesis.

Rafael being pulled to the Badestrasse after the graduation ceremony

Rafael Krämer - first graduated PhD Student from the CRC1348 IRTG

We are happy to announce that in December 2019 Rafael Krämer from the Rumpf lab was graduated as first PhD student from the CRC1348 IRTG. Congratulations Dr. Krämer and well done!

CRC1348 Retreat 2019 Oberwerries

CRC 1348 Retreat 2019 Oberwerries

The CRC has staged a successful and productive annual retreat at the Landesturnschule Oberwerries with lots of mental and some physical exercise. We are due to return fully equipped with sweat pants, trainers and shower gel in 2020!