Project B04

Membrane Remodeling and Severing During Dendrite Pruning in Drosophila Neurons

Neuronal morphogenesis and circuit formation involve both progressive events like growth of axons and dendrites, and regressive events such as elimination or degeneration of synapses or sometimes long stretches of neurites, collectively known as pruning. The rearrangement of cellular membranes and membrane interfaces is an important but little-understood aspect of pruning. Peripheral sensory neurons of Drosophila larvae prune their extensive larval dendrites at the onset of metamorphosis in an ecdysone-dependent manner through a degenerative mechanism. Dendrite pruning requires the loss of cell adhesion molecules (CAMs). The molecular mechanisms underlying CAM degradation are not well understood, and it is not clear which specific cellular interfaces must be remodeled to allow pruning, or how lack of interface remodeling interferes with dendrite pruning. We have identified membrane trafficking regulators required for pruning and CAM degradation. We propose to use these mutants to unravel the triggers and mechanisms of CAM degradation, to identify the affected membrane interfaces, and to identify the mechanisms by which interface remodeling contributes to pruning.

Rafael being pulled to the Badestrasse after the graduation ceremony

Rafael Krämer - first graduated PhD Student from the CRC1348 IRTG

We are happy to announce that in December 2019 Rafael Krämer from the Rumpf lab was graduated as first PhD student from the CRC1348 IRTG. Congratulations Dr. Krämer and well done!

CRC1348 Retreat 2019 Oberwerries

CRC 1348 Retreat 2019 Oberwerries

The CRC has staged a successful and productive annual retreat at the Landesturnschule Oberwerries with lots of mental and some physical exercise. We are due to return fully equipped with sweat pants, trainers and shower gel in 2020!