Project A11

Regulation of Plasma Membrane Supramolecular Functional Units by Late Endosomal Sorting and Transport

Cellular membranes segregate into local ordered microdomains (“rafts”), physical units which assemble and interconnect cooperating molecules and serve as plasma membrane signaling hubs. In particular, tetraspanin-enriched microdomains (TEM) have been implicated in adhesion events occurring during migration and invasion, in leukocyte extravasation, host/pathogen interaction, and in various signaling pathways. The plasma membrane in general and the tetraspanin-enriched microdomains in particular undergo dynamic rearrangements due to both constant and regulated endo- and exocytotic events, the impact of which is often underestimated. We have recently identified a novel layer of regulation for TEM-controlled cell surface processes that is exerted from endosomal compartments. Based on our findings, we hypothesize that late endosomes act as a tetraspanin reservoir and that complex TEM-based cellular functions at the plasma membrane (e.g. in leukocyte recruitment) are affected by tetraspanin trafficking from late endosomes to the plasma membrane. The goal of the project is to gain information on the biological significance of tetraspanin recruitment to late endosomes. We will link changes of late endosomal tetraspanin levels to functional TEM alterations at the cell surface, and we will unravel the molecular mechanisms whereby tetraspanins are directed from late endosomes to the plasma membrane.

Rafael being pulled to the Badestrasse after the graduation ceremony

Rafael Krämer - first graduated PhD Student from the CRC1348 IRTG

We are happy to announce that in December 2019 Rafael Krämer from the Rumpf lab was graduated as first PhD student from the CRC1348 IRTG. Congratulations Dr. Krämer and well done!

CRC1348 Retreat 2019 Oberwerries

CRC 1348 Retreat 2019 Oberwerries

The CRC has staged a successful and productive annual retreat at the Landesturnschule Oberwerries with lots of mental and some physical exercise. We are due to return fully equipped with sweat pants, trainers and shower gel in 2020!