Our lab investigates regressive events during neuronal morphogenesis, in particular, pruning, the regulated degeneration of neurites without loss of the parent neuron. Pruning is a very important mechanism during neuronal circuit formation, yet it is still incompletely understood at the cell biological level. We use Drosophila peripheral sensory c4da neurons as a model to study pruning. These neurons specifically prune their long and branched larval dendrites at the onset of metamorphosis in a manner depending on the molting hormone ecdysone. Dendrite pruning requires the local destruction of the dendritic cytoskeleton and the remodeling of plasma membrane composition and cell adhesion. We are particularly interested in the question how dendrite degeneration is triggered, and how severing sites are marked. Our recent results suggest that both of these processes are linked to the regulation of the microtubule cytoskeleton and plasma membrane trafficking. In a second line of investigation, we are interested in posttranscriptional gene regulation mechanisms during metamorphosis.
Herzmann S, Götzelmann I, Reekers LF, Rumpf S 2018.
Spatial regulation of microtubule disruption during dendrite pruning in Drosophila. Development 145(9). pii: dev156950.
Herzmann S, Krumkamp R, Rode S, Kintrup C, Rumpf S 2017.
PAR-1 promotes microtubule breakdown during dendrite pruning in Drosophila. EMBO J. 36(13):1981-1991.
Rumpf S, Bagley JA, Thompson-Peer KL, Zhu S, Gorczyca D, Beckstead RB, Jan LY, Jan YN 2014. Drosophila Valosin-Containing Protein is required for dendrite pruning through a regulatory role in mRNA metabolism.
Proc Natl Acad Sci U S A. 111(20):7331-6.
Rumpf S, Lee SB, Jan LY, Jan YN 2011. Neuronal remodeling and apoptosis require VCP-dependent degradation of the apoptosis inhibitor DIAP1.
Yang CH, Rumpf S, Xiang Y, Gordon MD, Song W, Jan LY, Jan YN 2009. Control of the postmating behavioral switch in Drosophila females by internal sensory neurons. Neuron 61(4):519-26.