Ivan Bedzhov

Research Topic

Our research aims to understand the mechanisms of early mammalian development before and at the time of implantation on cellular and molecular level. We use the mouse embryo as a model system to decipher the interconnected processes of self-organization and cell fate transitions of the early lineages. To study these basic properties of the mammalian conceptus we use embryo micromanipulation techniques, 3D stem cell culture, genetic and genomic engineering, next generation sequencing methods, advanced microscopy and automated functional screens. The project within the Collaborative Research Center focuses on the architectural reorganization of the trophoblast stem cell pool (extraembryonic ectoderm) of the implanting embryo. These cells are critical for establishing the connection to the maternal tissues during the early stages of pregnancy and later for the formation of the placenta. Our aim is to dissect the signaling circuits that regulate the shape and function of the extraembryonic ectoderm during the implantation process.

Publications

Bedzhov I, Bialecka M, Zielinska A, Kosalka J, Antonica F, Thompson AJ, Franze K, Zernicka-Goetz M – Development of the anterior-posterior axis is a self-organising process in the absence of maternal cues in the mouse embryo. Cell Research, 2015

Bedzhov I, Zernicka-Goetz M – Cell death and morphogenesis during early mouse development: are they interconnected? Bioessays, 2015

Bedzhov I, Stemmler MP – Applying the Proximity Ligation Assay (PLA) to mouse preimplantation embryos for identifying protein-protein interactions in situ. Methods in Molecular Biology, 2015

Bedzhov I, Zernicka-Goetz M – Self-organizing properties of mouse pluripotent cells initiate morphogenesis upon implantation. Cell, 2014

Bedzhov I, Bialecka M, Leung CY, Zernicka-Goetz M – In vitro culture of mouse blastocysts beyond the implantation stages. Nature Protocols, 2014

Bedzhov I, Leung CY, Graham S, Zernicka-Goetz M – Developmental plasticity, cell fate specification and morphogenesis in the early mouse embryo, Philosophical Transactions B, 2014

Bedzhov I, Alotaibi H, Basilicata MF, Ahlborn K, Liszewska E, Brabletz T, Stemmler MP – Adhesion, but not a specific cadherin code, is indispensable for ES cell and induced pluripotency. Stem Cell Research, 2013

Bedzhov I, Liszewska E, Kanzler B, Stemmler MP – Igf1r signaling is indispensable for preimplantation development and is activated via a novel function of E-cadherin. PLoS Genetics, 2012

Stemmler MP, Bedzhov I – A Cdh1HA knock-in allele rescues the Cdh1−/− phenotype but shows essential Cdh1 function during placentation. Developmental Dynamics, 2010

Borissova, T. Hvarleva, I. Bedzhov, V. Kondakova, A. Atanassov, I. Atanassov – Agrobacterium - mediated transformation of secondary somatic embryos from Rosa hybrida L. and recovery of transgenic plants. Biotech. & Biotech Eq. 2005

Patents

Media and methods for culturing embryos and stem cells. 2015, WO2015022541