Molecular parameters for the dynamic assembly of Hedgehog signaling platforms
Hedgehog (Hh) morphogens are important signaling molecules during embryonic development and tissue homeostasis. They are dually lipidated and tether to the plasma membrane after their secretion. On the cell surface Hhs form multimers, to which factors required for their release will be recruited. Another important factor known to be involved in Hh release is called Dispatched (Disp), but so far, the exact function of Disp is unknown.
In our group we use cell culture and the model organism Drosophila melanogaster to study the molecular mechanism that governs Hh platform assembly, structure and Hh release and the role of Disp in this process.
Approved Pilot Project 2019:
A technique to visualize specific cell-surface heparan sulfate proteoglycans in vivo in Drosophila melanogaster