Pilot Project 6 (2020)

Identifying regulators of late endosomal cholesterol homeostasis and post-endosomal trafficking routes using proximity proteomics

Late endosomes and lysosomes control intracellular trafficking routes by monitoring the cellular homeostasis of cholesterol. Cholesterol is taken up by cells via endocytosis and is distributed from late endosomal/lysosomal compartments to other cellular destinations. The regulation of the late endosomal cholesterol content is of central importance to several cellular processes including the control of virus infection and endothelial cell activation during inflammation. Increasing late endosomal cholesterol levels is part of the innate immune response to reduce influenza A virus infection by inhibiting viral escape from the endosome. At the same time, late endosomal cholesterol accumulation impairs endothelial cell activation by blocking protein trafficking from late endosomal compartments to Weibel-Palade Bodies (WPB) endothelial cell-specific secretory granules. To further investigate the impact of late endosomal cholesterol homeostasis on downstream trafficking routes, we plan to acquire and compare the associated proteomes of late endosomes and WPBs in control cells and cells with pharmacologically inhibited late endosomal cholesterol efflux via proximity-dependent labeling.

Rafael being pulled to the Badestrasse after the graduation ceremony

Rafael Krämer - first graduated PhD Student from the CRC1348 IRTG

We are happy to announce that in December 2019 Rafael Krämer from the Rumpf lab was graduated as first PhD student from the CRC1348 IRTG. Congratulations Dr. Krämer and well done!

CRC1348 Retreat 2019 Oberwerries

CRC 1348 Retreat 2019 Oberwerries

The CRC has staged a successful and productive annual retreat at the Landesturnschule Oberwerries with lots of mental and some physical exercise. We are due to return fully equipped with sweat pants, trainers and shower gel in 2020!