Pilot Project 3 (2019)

Mutations in VE-PTP: Identification of a Novel Cause of Venous Malformations in 3D-Matrices

Mutations in the vascular endothelial protein tyrosine phosphatase (VE-PTP), one of the most important interaction partners of angiopoietin receptor Tie-2, were recently discovered in eight patients suffering from venous malformations. The aim of this project is to validate the prelimi- nary results of two candidate mutations, acquired from COS7 cell experiments – an impaired ability to dephosphorylate Tie-2. Confirming these results in VE-PTP re-transfected primary endothelial cells will depict the first milestone and provide essential evidence to allow a subsequent set of main experiments: A microfluidics-based experiment in three-dimensional hydrogels is planned to reveal potential abnormal vessel formation as it occurs in VM patients.

The described project is targeted at utilizing patient derived mutations and a set of in vitro experiments designed for mimicking true physiological in vivo conditions, thus, reducing the number of further animal experiments. Only in case of positive results, the experiment conducted by Boscolo et al. will be repeated to validate the data in an in vivo system in future experiments.

Rafael being pulled to the Badestrasse after the graduation ceremony

Rafael Krämer - first graduated PhD Student from the CRC1348 IRTG

We are happy to announce that in December 2019 Rafael Krämer from the Rumpf lab was graduated as first PhD student from the CRC1348 IRTG. Congratulations Dr. Krämer and well done!

CRC1348 Retreat 2019 Oberwerries

CRC 1348 Retreat 2019 Oberwerries

The CRC has staged a successful and productive annual retreat at the Landesturnschule Oberwerries with lots of mental and some physical exercise. We are due to return fully equipped with sweat pants, trainers and shower gel in 2020!