Project A02

Local Signaling for Tight Coupling of Synaptic Exo- and Endocytosis

In the CNS communication between neurons occurs at synapses by secretion of neurotransmitter via exocytosis of synaptic vesicles (SVs). Given the limited number of SVs in presynaptic boutons a fast and efficient trafficking cycle is required. Within this CRC we use cultured mammalian hippocampal neurons in conjunction with high and superresolution imaging methods to understand how local membrane signaling platforms orchestrate resorting and recycling of exocytosed SV proteins and lipids for proper SV reformation: We aim at answering the following central questions: Do SV proteins / lipids in part remain clustered after exocytosis, thereby facilitating formation of pre-assembled endocytic structures at the endocytic zone? To what extent do self-assembly forces between SV proteins/lipids function in maintaining endocytic domains (readily retrievable pool at endocytic sites)? What couples so tightly exo- and compensatory endocytosis despite their clear separation in two membrane domains?

Rafael being pulled to the Badestrasse after the graduation ceremony

Rafael Krämer - first graduated PhD Student from the CRC1348 IRTG

We are happy to announce that in December 2019 Rafael Krämer from the Rumpf lab was graduated as first PhD student from the CRC1348 IRTG. Congratulations Dr. Krämer and well done!

CRC1348 Retreat 2019 Oberwerries

CRC 1348 Retreat 2019 Oberwerries

The CRC has staged a successful and productive annual retreat at the Landesturnschule Oberwerries with lots of mental and some physical exercise. We are due to return fully equipped with sweat pants, trainers and shower gel in 2020!